MC2R
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MRAP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The e3 ubiquitin ligase mahogunin ubiquitinates the melanocortin 2 receptor.
Mahogunin ring fnger-1(MGRN1) encodes an E3 ubiquitin ligase and is mutated in the mahoganoid mouse. The mahoganoid mouse mutant shows similarities to the phenotype of another spontaneous mouse mutation known as mahogunin (mutation in attractin) presenting with dark coat color, spongiform neurodegeneration, and high embryonic lethality. It has long been hypothesized that MGRN1 may down-regulate the function of the melanocortin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MC2R MRAP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2826831 |
Curated By
- BioGRID