POL3
Gene Ontology Biological Process
- DNA replication [IMP]
- DNA replication proofreading [IBA]
- DNA replication, removal of RNA primer [IDA]
- DNA-dependent DNA replication maintenance of fidelity [IGI]
- RNA-dependent DNA replication [IDA]
- base-excision repair, gap-filling [IBA]
- nucleotide-excision repair, DNA gap filling [IBA]
- regulation of mitotic cell cycle [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MRC1
Gene Ontology Biological Process
- DNA repair [IGI, IMP]
- DNA replication [IMP]
- DNA replication checkpoint [IGI, IMP, IPI]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- chromatin silencing at telomere [IGI, IMP]
- intra-S DNA damage checkpoint [IMP]
- maintenance of DNA repeat elements [IMP]
- mitotic sister chromatid cohesion [IGI, IMP]
- regulation of nuclear cell cycle DNA replication [IMP]
- replication fork protection [IGI, IMP, IPI]
- telomere maintenance [IMP]
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Drug-sensitive DNA Polymerase {delta} Reveals a Role for Mismatch Repair in Checkpoint Activation in Yeast.
We have used a novel method to activate the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae - to slow lagging strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase δ (L612M-DNA pol δ) to the inhibitory drug phosphonoacetic acid (PAA). PAA-treated pol3-L612M cells arrest as large budded cells with a single nucleus in the bud neck. This ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- pol3-L612M mrc1 double mutant has increased sensitivity to PAA
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POL3 MRC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1557 | BioGRID | 364046 | |
| MRC1 POL3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2373 | BioGRID | 2030925 | |
| POL3 MRC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.4769 | BioGRID | 1965049 | |
| POL3 MRC1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 111757 |
Curated By
- BioGRID