BAIT

POL3

CDC2, HPR6, TEX1, DNA-directed DNA polymerase delta POL3, L000000242, YDL102W
Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
Saccharomyces cerevisiae (S288c)
PREY

SML1

ribonucleotide reductase inhibiting protein SML1, L000004580, YML058W
Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Drug-sensitive DNA Polymerase {delta} Reveals a Role for Mismatch Repair in Checkpoint Activation in Yeast.

Reha-Krantz LJ, Siddique MS, Murphy K, Tam A, O'Carroll M, Lou S, Schultz A, Boone C

We have used a novel method to activate the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae - to slow lagging strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase δ (L612M-DNA pol δ) to the inhibitory drug phosphonoacetic acid (PAA). PAA-treated pol3-L612M cells arrest as large budded cells with a single nucleus in the bud neck. This ... [more]

Unknown Sep. 16, 2011; 0(0); [Pubmed: 21926300]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • pol3-L612M mec1 sml1 triple mutant has increased sensitivity to PAA compared to pol3-L612M single and mec1 sml1 double mutants

Curated By

  • BioGRID