BAIT
GLIS2
NKL, NPHP7
GLIS family zinc finger 2
GO Process (6)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- negative regulation of sequence-specific DNA binding transcription factor activity [ISS]
- negative regulation of smoothened signaling pathway [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
WNK1
HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65
WNK lysine deficient protein kinase 1
GO Process (7)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
The Krueppel-like zinc finger protein Glis2 functions as a negative modulator of the Wnt/beta-catenin signaling pathway.
To gain insight into the mechanism by which Gli-similar 2 (Glis2) regulates transcription, we performed yeast-two hybrid cDNA library screening using Glis2 as bait. This screening identified beta-catenin as a potential Glis2-interacting protein. Mammalian two-hybrid, co-immunoprecipitation, and GST-pulldown analyses supported the interaction between Glis2 and beta-catenin. Pulldown analyses with several Glis2 deletion mutants indicated that the 1st zinc finger motif ... [more]
FEBS Lett. Mar. 06, 2007; 581(5);858-64 [Pubmed: 17289029]
Throughput
- Low Throughput
Curated By
- BioGRID