BAIT

FUS6

ARABIDOPSIS THALIANA FUSCA 6, ATFUS6, ATSK31, CONSTITUTIVE PHOTOMORPHOGENIC 11, COP11, COP9 SIGNALOSOME SUBUNIT 1, CSN1, EMB78, EMBRYO DEFECTIVE 78, FUSCA 6, SHAGGY-LIKE KINASE 31, SK31, AT3G61140
COP9 signalosome complex subunit 1
Arabidopsis thaliana (Columbia)
PREY

COP13

CONSTITUTIVE PHOTOMORPHOGENIC 13, COP9 SIGNALOSOME SUBUNIT 3, CSN3, F18O22.40, F18O22_40, FUS11, FUSCA 11, AT5G14250
COP9 signalosome complex subunit 3
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TSA1 interacts with CSN1/CSN and may be functionally involved in Arabidopsis seedling development in darkness.

Li W, Zang B, Liu C, Lu L, Wei N, Cao K, Deng XW, Wang X

The COP9 signalosome (CSN) is a multiprotein complex which participates in diverse cellular and developmental processes. CSN1, one of the subunits of CSN, is essential for assembly of the multiprotein complex via PCI (proteasome, COP9 signalosome and initiation factor 3) domain in the C-terminal half of CSN1. However, the role of the N-terminal domain (NTD) of CSN1, which is critical ... [more]

J Genet Genomics Nov. 20, 2011; 38(11);539-46 [Pubmed: 22133685]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COP13 FUS6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
COP13 FUS6
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-BioGRID
-
COP13 FUS6
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.8852BioGRID
2912833
COP13 FUS6
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-BioGRID
-
COP13 FUS6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID