An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Arabidopsis Tail-Anchored Protein PEROXISOMAL AND MITOCHONDRIAL DIVISION FACTOR1 Is Involved in the Morphogenesis and Proliferation of Peroxisomes and Mitochondria.

Aung K, Hu J

Peroxisomes and mitochondria are multifunctional eukaryotic organelles that are not only interconnected metabolically but also share proteins in division. Two evolutionarily conserved division factors, dynamin-related protein (DRP) and its organelle anchor FISSION1 (FIS1), mediate the fission of both peroxisomes and mitochondria. Here, we identified and characterized a plant-specific protein shared by these two types of organelles. The Arabidopsis thaliana PEROXISOMAL ... [more]

Unknown Dec. 06, 2011; 0(0); [Pubmed: 22147290]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT1G06530 AT3G58840	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Aung K (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT1G06530

Gene Ontology Biological Process

Gene Ontology Cellular Component

AT3G58840