BAIT

RIC8B

BC051080, Ric-8, Ric-8b
resistance to inhibitors of cholinesterase 8 homolog B (C. elegans)
GO Process (2)
GO Function (3)
GO Component (3)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Ric-8B stabilizes the alpha subunit of stimulatory G protein by inhibiting its ubiquitination.

Nagai Y, Nishimura A, Tago K, Mizuno N, Itoh H

The alpha subunit of stimulatory G protein (G alpha(s)) activates adenylyl cyclase, which catalyzes cAMP production, and regulates many physiological aspects, such as cardiac regulation and endocrine systems. Ric-8B (resistance to inhibitors of cholinesterase 8B) has been identified as the G alpha(s)-binding protein; however, its role in G(s) signaling remains obscure. In this study, we present evidence that Ric-8B specifically ... [more]

J. Biol. Chem. Apr. 09, 2010; 285(15);11114-20 [Pubmed: 20133939]

Throughput

  • Low Throughput

Curated By

  • BioGRID