BAIT

HLA-A

HLAA, DAQB-90C11.16-002
major histocompatibility complex, class I, A
GO Process (17)
GO Function (7)
GO Component (15)
Homo sapiens
PREY

PSMD2

P97, RPN1, S2, TRAP2
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
GO Process (21)
GO Function (1)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation.

Burr ML, Cano F, Svobodova S, Boyle LH, Boname JM, Lehner PJ

The assembly of MHC class I molecules is governed by stringent endoplasmic reticulum (ER) quality control mechanisms. MHC class I heavy chains that fail to achieve their native conformation in complex with β2-microglobulin (β2m) and peptide are targeted for ER-associated degradation. This requires ubiquitination of the MHC class I heavy chain and its dislocation from the ER to the cytosol ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 01, 2011; 108(5);2034-9 [Pubmed: 21245296]

Throughput

  • Low Throughput

Curated By

  • BioGRID