MKL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
SRF
Gene Ontology Biological Process
- angiogenesis involved in wound healing [TAS]
- cell migration involved in sprouting angiogenesis [IMP]
- cellular senescence [IMP]
- heart development [ISS]
- heart looping [ISS]
- mRNA transcription from RNA polymerase II promoter [ISS]
- muscle cell cellular homeostasis [ISS]
- negative regulation of beta-amyloid clearance [IMP]
- neuron development [TAS]
- positive regulation of cell differentiation [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of smooth muscle contraction [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [IGI]
- positive regulation of transcription initiation from RNA polymerase II promoter [IDA]
- positive regulation of transcription via serum response element binding [IDA]
- regulation of smooth muscle cell differentiation [TAS]
- response to cytokine [IMP, NAS]
- response to hormone [IDA]
- response to hypoxia [IEP]
- response to toxic substance [TAS]
- transcription from RNA polymerase II promoter [IDA]
- trophectodermal cell differentiation [IDA]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- serum response element binding [IDA]
- transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- serum response element binding [IDA]
- transcription factor binding [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
β-Catenin and Smad3 regulate the activity and stability of myocardin-related transcription factor during epithelial-myofibroblast transition.
Injury to the adherens junctions (AJs) synergizes with transforming growth factor-β1 (TGFβ) to activate a myogenic program (α-smooth muscle actin [SMA] expression) in the epithelium during epithelial-myofibroblast transition (EMyT). Although this synergy plays a key role in organ fibrosis, the underlying mechanisms have not been fully defined. Because we recently showed that Smad3 inhibits myocardin-related transcription factor (MRTF), the driver ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MKL1 SRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MKL1 SRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SRF MKL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SRF MKL1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID