IRF2
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell proliferation [TAS]
- cytokine-mediated signaling pathway [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription, DNA-templated [IMP]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IMP]
- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IMP]
Gene Ontology Cellular Component
- cytoplasm [IDA]
- cytosol [TAS]
- focal adhesion [IDA]
- nucleoplasm [IDA, TAS]
ATG7
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- cellular protein modification process [TAS]
- cellular response to hyperoxia [IDA]
- cellular response to nitrogen starvation [IBA]
- cellular response to starvation [IDA]
- late nucleophagy [IBA]
- membrane fusion [TAS]
- mitochondrion degradation [IBA]
- piecemeal microautophagy of nucleus [IBA]
- positive regulation of apoptotic process [IMP]
- positive regulation of autophagy [IMP]
- positive regulation of macroautophagy [IMP]
- positive regulation of protein catabolic process [IMP]
- positive regulation of protein modification process [IDA]
- protein catabolic process [IBA]
- protein lipidation [IDA]
- protein modification by small protein conjugation [IBA]
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
To systematically investigate innate immune signaling networks regulating production of type I interferon, we analyzed protein complexes formed after microbial recognition. Fifty-eight baits were associated with 260 interacting proteins forming a human innate immunity interactome for type I interferon (HI5) of 401 unique interactions; 21% of interactions were modulated by RNA, DNA, or LPS. Overexpression and depletion analyses identified 22 ... [more]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007)
Additional Notes
- exogenous expression of bait
- some interactions identified in this screen were stimulated by the addition of poly(rI:rC), poly(dA:dT), LPS, and/or CpG
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRF2 ATG7 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID