BAIT
IRF4
LSIRF, MUM1, NF-EM5, SHEP8
interferon regulatory factor 4
GO Process (13)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- T cell activation [NAS]
- T-helper 17 cell lineage commitment [ISS]
- cytokine-mediated signaling pathway [TAS]
- defense response to protozoan [ISS]
- interferon-gamma-mediated signaling pathway [TAS]
- positive regulation of interleukin-10 biosynthetic process [IDA]
- positive regulation of interleukin-13 biosynthetic process [IDA]
- positive regulation of interleukin-2 biosynthetic process [IDA]
- positive regulation of interleukin-4 biosynthetic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of T-helper cell differentiation [NAS]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [NAS]
Homo sapiens
PREY
VPS35
MEM3, PARK17, TCCCTA00141
vacuolar protein sorting 35 homolog (S. cerevisiae)
GO Process (5)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
To systematically investigate innate immune signaling networks regulating production of type I interferon, we analyzed protein complexes formed after microbial recognition. Fifty-eight baits were associated with 260 interacting proteins forming a human innate immunity interactome for type I interferon (HI5) of 401 unique interactions; 21% of interactions were modulated by RNA, DNA, or LPS. Overexpression and depletion analyses identified 22 ... [more]
Immunity Sep. 23, 2011; 35(3);426-40 [Pubmed: 21903422]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007)
Additional Notes
- exogenous expression of bait
- some interactions identified in this screen were stimulated by the addition of poly(rI:rC), poly(dA:dT), LPS, and/or CpG
Curated By
- BioGRID