BAIT
EIF2AK2
EIF2AK1, PKR, PPP1R83, PRKR
eukaryotic translation initiation factor 2-alpha kinase 2
GO Process (22)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- activation of MAPKK activity [IMP]
- evasion or tolerance by virus of host immune response [TAS]
- modulation by virus of host morphology or physiology [TAS]
- modulation by virus of host process [TAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of osteoblast proliferation [IMP]
- negative regulation of translation [IDA, IMP]
- negative regulation of viral genome replication [IMP]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of NIK/NF-kappaB signaling [ISS]
- positive regulation of chemokine production [ISS]
- positive regulation of cytokine production [ISS]
- positive regulation of stress-activated MAPK cascade [ISS]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA]
- regulation of NLRP3 inflammasome complex assembly [ISS]
- regulation of hematopoietic progenitor cell differentiation [ISS]
- regulation of hematopoietic stem cell differentiation [ISS]
- regulation of hematopoietic stem cell proliferation [ISS]
- response to interferon-alpha [IDA]
- response to virus [IMP]
- viral life cycle [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
HNRNPCL1
HNRPCL1, RP5-845O24.4
heterogeneous nuclear ribonucleoprotein C-like 1
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
To systematically investigate innate immune signaling networks regulating production of type I interferon, we analyzed protein complexes formed after microbial recognition. Fifty-eight baits were associated with 260 interacting proteins forming a human innate immunity interactome for type I interferon (HI5) of 401 unique interactions; 21% of interactions were modulated by RNA, DNA, or LPS. Overexpression and depletion analyses identified 22 ... [more]
Immunity Sep. 23, 2011; 35(3);426-40 [Pubmed: 21903422]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- exogenous expression of bait
- some interactions identified in this screen were stimulated by the addition of poly(rI:rC), poly(dA:dT), LPS, and/or CpG
Curated By
- BioGRID