Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A directed protein interaction network for investigating intracellular signal transduction.

Vinayagam A, Stelzl U, Foulle R, Plassmann S, Zenkner M, Timm J, Assmus HE, Andrade-Navarro MA, Wanker EE

Cellular signal transduction is a complex process involving protein-protein interactions (PPIs) that transmit information. For example, signals from the plasma membrane may be transduced to transcription factors to regulate gene expression. To obtain a global view of cellular signaling and to predict potential signal modulators, we searched for protein interaction partners of more than 450 signaling-related proteins by means of ... [more]

Sci Signal Sep. 06, 2011; 4(189);rs8 [Pubmed: 21900206]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

NUDT21

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]histone deacetylase binding [IPI]mRNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

PTN