BAIT

CDC27

cell division cycle 27
GO Process (2)
GO Function (1)
GO Component (6)
Rattus norvegicus
PREY

KRAS

Kras2, c-Ki-ras, p21
Kirsten rat sarcoma viral oncogene
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Degradation of p21Cip1 through APC/CCdc20 mediated ubiquitylation is inhibited by cyclin dependent kinase 2 in cardiomyocytes.

Yamada K, Tamamori-Adachi M, Goto I, Iizuka M, Yasukawa T, Aso T, Okazaki T, Kitajima S

Cyclin dependent kinase (CDK) inhibitor p21Cip1 plays a crucial role in regulating cell cycle arrest and differentiation. It is known that p21Cip1 increases during terminal differentiation of cardiomyocytes, but its expression control and biological role are not fully understood. Here we show that p21Cip1 protein is stabilized in cardiomyocytes after mitogenic stimulation, due to its increased CDK2 binding and inhibition ... [more]

Unknown Nov. 01, 2011; 0(0); [Pubmed: 22045811]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KRAS CDC27
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID