Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA.

Dantonel JC, Murthy KG, Manley JL, Tora L

Initiation of transcription by RNA polymerase II from a promoter region on DNA requires the assembly of several initiation factors to form a preinitiation complex. Assembly of this complex is initiated by the binding of the transcription factor TFIID, composed of the TATA-box binding protein (TBP) and TBP-associated factors (TAF[II]s), to the promoter. We have now characterized an immunopurified TFIID ... [more]

Nature Sep. 25, 1997; 389(6649);399-402 [Pubmed: 9311784]

Throughput

  • Low Throughput

Curated By

  • BioGRID