RNF8
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IDA, ISO]
- double-strand break repair [ISO]
- double-strand break repair via nonhomologous end joining [IMP]
- histone H2A K63-linked ubiquitination [ISO]
- histone H2A ubiquitination [IMP, ISO]
- histone H2B ubiquitination [IMP]
- histone exchange [IMP]
- isotype switching [IMP]
- negative regulation of transcription elongation from RNA polymerase II promoter [ISO]
- positive regulation of DNA repair [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- response to ionizing radiation [ISO]
- spermatid development [IMP]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TPP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection.
The mammalian shelterin component TPP1 has essential roles in telomere maintenance and, together with POT1, is required for the repression of DNA damage signaling at telomeres. Here we show that in Mus musculus, the E3 ubiquitin ligase Rnf8 localizes to uncapped telomeres and promotes the accumulation of DNA damage proteins 53Bp1 and γ-H2ax. In the absence of Rnf8, Tpp1 is ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
TPP1 RNF8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
RNF8 TPP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID