ITSN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SPRY2
Gene Ontology Biological Process
- branching morphogenesis of an epithelial tube [IMP]
- bud elongation involved in lung branching [IGI]
- cell fate commitment [IGI, IMP]
- establishment of mitotic spindle orientation [IGI]
- inner ear morphogenesis [IGI, IMP]
- lung development [IMP]
- lung growth [IDA]
- lung morphogenesis [IDA]
- negative regulation of ERK1 and ERK2 cascade [IDA, IGI]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of Ras GTPase activity [IDA]
- negative regulation of Ras protein signal transduction [IDA]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cell proliferation [IDA]
- negative regulation of fibroblast growth factor receptor signaling pathway [IDA, IGI]
- negative regulation of neurotrophin TRK receptor signaling pathway [IDA]
- negative regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- respiratory system development [IDA]
- sensory perception of sound [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Intersectin 1 enhances Cbl ubiquitylation of epidermal growth factor receptor through regulation of Sprouty2-Cbl interaction.
Ubiquitylation of receptor tyrosine kinases plays a critical role in regulating the trafficking and lysosomal degradation of these important signaling molecules. We identified the multi-domain scaffolding protein intersectin 1 (ITSN1) as an important regulator of this process. ITSN1 stimulates ubiquitylation of the epidermal growth factor receptor (EGFR) through enhancing the activity of the Cbl E3 ubiquitin ligase. However, the precise ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITSN1 SPRY2 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | - | |
| ITSN1 SPRY2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID