KLHL2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CUL3
Gene Ontology Biological Process
- COPII vesicle coating [ISO]
- ER to Golgi vesicle-mediated transport [ISO]
- Wnt signaling pathway [IDA]
- cell migration [ISO]
- cell morphogenesis [IMP]
- embryonic cleavage [IMP]
- fibroblast apoptotic process [IMP]
- gastrulation [IMP]
- in utero embryonic development [IMP]
- integrin-mediated signaling pathway [IMP]
- liver morphogenesis [IMP]
- mitotic cell cycle [IMP]
- mitotic metaphase plate congression [ISO]
- negative regulation of Rho protein signal transduction [ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of cytokinesis [ISO]
- positive regulation of mitotic metaphase/anaphase transition [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IGI, ISO]
- protein monoubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [ISO]
- regulation of transcription from RNA polymerase II promoter [IGI]
- stem cell division [IMP]
- stress fiber assembly [ISO]
- trophectodermal cellular morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of an intracellular pentraxin with a BTB-Kelch protein is associated with ubiquitylation, aggregation and neuronal apoptosis.
Neuronal pentraxin with chromo domain (NPCD) comprises a group of neuronally expressed pentraxins with both membrane and cytosolic isoforms; the functions of cytosolic NPCD isoforms are not clear. Here, we demonstrate that a cytosolic NPCD isoform selectively interacts with the BTB-Kelch protein Mayven/Kelch-like 2 (KLHL2), an actin-binding protein implicated in process outgrowth in oligodendrocytes. The KLHL2-NPCD interaction was identified by ... [more]
Throughput
- Low Throughput
Additional Notes
- source of Cul3 not clear
Curated By
- BioGRID