Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Ataxin-3 deubiquitination is coupled to parkin ubiquitination via the E2 ubiquitin-conjugating enzyme.

Durcan TM, Kontogiannea M, Bedard N, Wing SS, Fon EA

We reported previously that parkin, a Parkinson's disease-associated E3 ubiquitin-ligase interacts with ataxin-3, a deubiquitinating enzyme (DUB) associated with Machado-Joseph disease. Ataxin-3 was found to counteract parkin self-ubiquitination both in vitro and in cells. Moreover, ataxin-3-dependent deubiquitination of parkin required the catalytic cysteine 14 in ataxin-3, although the precise mechanism remained unclear. We report here that ataxin-3 interferes with ... [more]

Unknown Nov. 11, 2011; 0(0); [Pubmed: 22081612]

Throughput

  • Low Throughput

Curated By

  • BioGRID