BAIT

VCP

3110001E05, CDC48, p97, p97/VCP, RP23-124L1.5
valosin containing protein
GO Process (19)
GO Function (13)
GO Component (15)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ER-stress-inducible Herp, facilitates the degradation of immature nicastrin.

Marutani T, Maeda T, Tanabe C, Zou K, Araki W, Kokame K, Michikawa M, Komano H

Herp is an endoplasmic reticulum (ER)-stress-inducible membrane protein harboring an ubiquitin-like domain (ULD). However, its biological functions are not fully understood. Here, we examined the role of Herp in the degradation of γ-secretase components.Effects of ULD-lacking Herp (ΔUb-Herp) expression on the degradation of γ-secretase components were analyzed.The cellular expression of ΔUb-Herp was found to inhibit the degradation of overexpressed immature ... [more]

Biochim. Biophys. Acta Aug. 01, 2011; 1810(8);790-8 [Pubmed: 21600962]

Throughput

  • Low Throughput

Additional Notes

  • source of Herp not clear

Curated By

  • BioGRID