An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CSPÎ± promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.

Sharma M, Burre J, Suedhof TC

A neuron forms thousands of presynaptic nerve terminals on its axons, far removed from the cell body. The protein CSPÎ± resides in presynaptic terminals, where it forms a chaperone complex with Hsc70 and SGT. Deletion of CSPÎ± results in massive neurodegeneration that impairs survival in mice and flies. In CSPÎ±-knockout mice, levels of presynaptic SNARE complexes and the SNARE protein ... [more]

Nat. Cell Biol. Jan. 01, 2011; 13(1);30-9 [Pubmed: 21151134]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNAP25 SNCA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Burre J (2010)	Low	-	BioGRID	727275
SNCA SNAP25	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Burre J (2010)	Low	-	BioGRID	727278
SNAP25 SNCA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sharma M (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SNCA

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

SNAP25