BAIT

HSPA8

2410008N15Rik, Hsc70, Hsc71, Hsc73, Hsp73, Hspa10
heat shock protein 8
Mus musculus
PREY

HSPA8

2410008N15Rik, Hsc70, Hsc71, Hsc73, Hsp73, Hspa10
heat shock protein 8
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.

Sharma M, Burre J, Suedhof TC

A neuron forms thousands of presynaptic nerve terminals on its axons, far removed from the cell body. The protein CSPα resides in presynaptic terminals, where it forms a chaperone complex with Hsc70 and SGT. Deletion of CSPα results in massive neurodegeneration that impairs survival in mice and flies. In CSPα-knockout mice, levels of presynaptic SNARE complexes and the SNARE protein ... [more]

Nat. Cell Biol. Jan. 01, 2011; 13(1);30-9 [Pubmed: 21151134]

Throughput

  • Low Throughput

Curated By

  • BioGRID