NEDD4
Gene Ontology Biological Process
- T cell activation [IMP]
- adaptive immune response [IMP]
- blood vessel morphogenesis [IGI, IMP]
- endocardial cushion development [IGI, IMP]
- negative regulation of sodium ion transport [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [IDA]
- neuromuscular junction development [IMP]
- outflow tract morphogenesis [IGI, IMP]
- positive regulation of protein catabolic process [IDA, ISO]
- protein K63-linked ubiquitination [ISO]
- protein monoubiquitination [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, IMP]
- regulation of dendrite morphogenesis [IMP, ISO]
- regulation of synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SCNN1A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical plasma membrane [ISO]
- ciliary membrane [ISO]
- cortical actin cytoskeleton [ISO]
- cytosol [IDA]
- external side of plasma membrane [IDA]
- extracellular vesicular exosome [ISO]
- integral component of membrane [IDA]
- integral component of plasma membrane [ISO]
- membrane [IDA]
- motile cilium [ISO]
- sodium channel complex [IGI, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
An obligatory heterodimer of 14-3-3beta and 14-3-3epsilon is required for aldosterone regulation of the epithelial sodium channel.
Increased distal nephron sodium absorption in response to aldosterone involves Nedd4-2 phosphorylation, which blocks its ability to ubiquitylate ENaC and increases apical membrane channel density by reducing its endocytosis. Our prior work (Liang, X., Peters, K. W., Butterworth, M. B., and Frizzell, R. A. (2006) J. Biol. Chem. 281, 16323-16332) showed that aldosterone selectively increased 14-3-3 protein isoform expression and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NEDD4 SCNN1A | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.796 | BioGRID | 2685918 | |
| SCNN1A NEDD4 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| NEDD4 SCNN1A | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | 696447 |
Curated By
- BioGRID