SMAD2
Gene Ontology Biological Process
- SMAD protein complex assembly [IDA]
- activin receptor signaling pathway [IMP]
- anterior/posterior pattern specification [ISS]
- cell fate commitment [ISS]
- common-partner SMAD protein phosphorylation [IDA]
- gastrulation [TAS]
- gene expression [TAS]
- intracellular signal transduction [ISS]
- mesoderm formation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- nodal signaling pathway [IMP]
- palate development [ISS]
- paraxial mesoderm morphogenesis [ISS]
- positive regulation of BMP signaling pathway [IMP]
- positive regulation of epithelial to mesenchymal transition [ISS]
- positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS, TAS]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISS]
- primary miRNA processing [TAS]
- regulation of binding [ISS]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to cholesterol [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [IPI]
- activating transcription factor binding [IPI]
- co-SMAD binding [IPI]
- double-stranded DNA binding [ISS]
- enhancer binding [IC]
- phosphatase binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [IPI]
- activating transcription factor binding [IPI]
- co-SMAD binding [IPI]
- double-stranded DNA binding [ISS]
- enhancer binding [IC]
- phosphatase binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
USP9X
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- axon extension [IMP]
- female gamete generation [TAS]
- gene expression [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- neuron migration [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IMP, TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
FAM/USP9x, a deubiquitinating enzyme essential for TGFbeta signaling, controls Smad4 monoubiquitination.
The assembly of the Smad complex is critical for TGFbeta signaling, yet the mechanisms that inactivate or empower nuclear Smad complexes are less understood. By means of siRNA screen we identified FAM (USP9x), a deubiquitinase acting as essential and evolutionarily conserved component in TGFbeta and bone morphogenetic protein signaling. Smad4 is monoubiquitinated in lysine 519 in vivo, a modification that ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID