BAIT

EP300

KAT3B, RSTS2, p300, RP1-85F18.1
E1A binding protein p300
GO Process (29)
GO Function (15)
GO Component (3)

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

p300/CBP acts as a coactivator of the cone-rod homeobox transcription factor.

Yanagi Y, Masuhiro Y, Mori M, Yanagisawa J, Kato S

CRX (cone-rod homeobox) is shown to play an important role on the photoreceptor cell maturation and maintenance. However, little is known about the molecular mechanism how CRX potentiates transcription. Here, we investigated the effect of several coactivators (p300/CBP, SRC-1, TIF2, and AIB1) on the transactivation function of CRX. The transient expression assay with a luciferase reporter gene showed that only ... [more]

Biochem. Biophys. Res. Commun. Mar. 16, 2000; 269(2);410-4 [Pubmed: 10708567]

Throughput

  • Low Throughput

Additional Notes

  • mammalian 2-hybrid

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CRX EP300
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3651723

Curated By

  • BioGRID