BAIT

SHY2

F19P19.32, F19P19_32, IAA3, SHORT HYPOCOTYL 2, indole-3-acetic acid inducible 3, AT1G04240
auxin-responsive protein IAA3
GO Process (1)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AFB2

auxin signaling F-box 2, AT3G26810
auxin signaling F-box 2
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Nitric Oxide Influences Auxin Signaling Through S-Nitrosylation of the Arabidopsis Transport Inhibitor Response1 Auxin Receptor.

Terrile MC, Paris R, Calderon-Villalobos LI, Iglesias MJ, Lamattina L, Estelle M, Casalongue CA

Previous studies demonstrated that auxin and nitric oxide (NO) are plant growth regulators that coordinate several plant physiological responses determining root architecture. Nonetheless, the way in which these factors interact to affect these growth and developmental processes is not well understood. The Arabidopsis thaliana F-box proteins TIR1/AFBs are auxin receptors that mediate degradation of Aux/IAA repressors to induce auxin-regulated responses. ... [more]

Unknown Dec. 15, 2011; 0(0); [Pubmed: 22171938]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AFB2 SHY2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID