An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Masking of a nuclear signal motif by monoubiquitination leads to mislocalization and degradation of the regulatory enzyme cytidylyltransferase.

Chen BB, Mallampalli RK

Monoubiquitination aids in the nuclear export and entrance of proteins into the lysosomal degradative pathway, although the mechanisms are unknown. Cytidylyltransferase (CCTalpha) is a proteolytically sensitive lipogenic enzyme containing an NH(2)-terminal nuclear localization signal (NLS). We show here that CCTalpha is monoubiquitinated at a molecular site (K(57)) juxtaposed near its NLS, resulting in disruption of its interaction with importin-alpha, nuclear ... [more]

Mol. Cell. Biol. Jun. 01, 2009; 29(11);3062-75 [Pubmed: 19332566]

Throughput

Low Throughput

Additional Notes

source of CCTalpha not clear
source of importin not clear

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PCYT1A KPNA2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Chen BB (2009)	Low	-	BioGRID	-
PCYT1A KPNA2	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Pourhaghighi R (2020)	High	0.933	BioGRID	2681786

Curated By

BioGRID

Interaction Summary

KPNA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase binding [ISO]nuclear localization sequence binding [ISO]poly(A) RNA binding [ISO]protein binding [IPI]protein transporter activity [IDA]

Gene Ontology Cellular Component

PCYT1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

choline-phosphate cytidylyltransferase activity [ISO]lipid binding [ISO]protein binding [IPI]protein homodimerization activity [ISO]