Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Calmodulin is a phospholipase C-beta interacting protein.

McCullar JS, Larsen SA, Millimaki RA, Filtz TM

Phospholipase C-beta 3 (PLC beta 3) is an important effector enzyme in G protein-coupled signaling pathways. Activation of PLC beta 3 by G alpha and G beta gamma subunits has been fairly well characterized, but little is known about other protein interactions that may also regulate PLC beta 3 function. A yeast two-hybrid screen of a mouse brain cDNA library ... [more]

J. Biol. Chem. Sep. 05, 2003; 278(36);33708-13 [Pubmed: 12821674]

Throughput

  • Low Throughput

Curated By

  • BioGRID