Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.

Rauch S, Martin-Serrano J

Late domains are short peptide sequences encoded by enveloped viruses to promote the final separation of the nascent virus from the infected cell. These amino acid motifs facilitate viral egress by interacting with components of the ESCRT (endosomal sorting complex required for transport) machinery, ultimately leading to membrane scission by recruiting ESCRT-III to the site of viral budding. PPXY late ... [more]

J. Virol. Apr. 01, 2011; 85(7);3546-56 [Pubmed: 21191027]

Throughput

  • Low Throughput

Curated By

  • BioGRID