Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Characterization of ubiquitination dependent dynamics in growth factor receptor signaling by quantitative proteomics.

Akimov V, Rigbolt KT, Nielsen MM, Blagoev B

Protein ubiquitination is a dynamic reversible post-translational modification that plays a key role in the regulation of numerous cellular processes including signal transduction, endocytosis, cell cycle control, DNA repair and gene transcription. The conjugation of the small protein ubiquitin or chains of ubiquitin molecules of various types and lengths to targeted proteins is known to alter proteins' lifespan, localization and ... [more]

Mol Biosyst Dec. 01, 2011; 7(12);3223-33 [Pubmed: 21956701]

Throughput

  • Low Throughput

Curated By

  • BioGRID