PCNA
Gene Ontology Biological Process
- DNA repair [TAS]
- DNA strand elongation involved in DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- base-excision repair [TAS]
- cell proliferation [TAS]
- epithelial cell differentiation [IEP]
- leading strand elongation [IBA]
- mismatch repair [IDA]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- positive regulation of deoxyribonuclease activity [IDA]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
- translesion synthesis [IDA]
Gene Ontology Molecular Function- DNA polymerase binding [IPI]
- DNA polymerase processivity factor activity [IBA]
- MutLalpha complex binding [IDA]
- dinucleotide insertion or deletion binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- purine-specific mismatch base pair DNA N-glycosylase activity [IDA]
- receptor tyrosine kinase binding [IPI]
- DNA polymerase binding [IPI]
- DNA polymerase processivity factor activity [IBA]
- MutLalpha complex binding [IDA]
- dinucleotide insertion or deletion binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- purine-specific mismatch base pair DNA N-glycosylase activity [IDA]
- receptor tyrosine kinase binding [IPI]
Gene Ontology Cellular Component
EXO1
Gene Ontology Biological Process
Gene Ontology Molecular Function- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Characterization of human exonuclease 1 in complex with mismatch repair proteins, subcellular localization and association with PCNA.
Human exonuclease 1 (hEXO1) has been implicated in DNA mismatch repair (MMR), replication, and recombination, but the nature of its interaction with these cellular processes is still ambiguous. We show that hEXO1 colocalizes with proliferating cell nuclear antigen (PCNA) at DNA replication sites and that the C-terminal region of hEXO1 is sufficient for this localization. We also show that both ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EXO1 PCNA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EXO1 PCNA | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| PCNA EXO1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| EXO1 PCNA | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID