CD40
Gene Ontology Biological Process
- B cell proliferation [NAS]
- cellular calcium ion homeostasis [IMP]
- cellular response to mechanical stimulus [IEP]
- platelet activation [NAS]
- positive regulation of Cdc42 GTPase activity [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IEP]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of Rac GTPase activity [IMP]
- positive regulation of endothelial cell apoptotic process [IDA]
- positive regulation of protein kinase C signaling [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of tyrosine phosphorylation of Stat1 protein [IMP]
- protein complex assembly [TAS]
- regulation of immune response [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSPA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes.
Tumor and viral antigens elicit a potent immune response by heat shock protein-dependent uptake of antigenic peptide with subsequent presentation by MHC I. Receptors on antigen-presenting cells that specifically bind and internalize a heat shock protein-peptide complex have not yet been identified. Here, we show that cells expressing CD40, a cell surface protein crucial for B cell function and autoimmunity, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CD40 HSPA4 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID