An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3.

Macdonald N, Welburn JP, Noble ME, Nguyen A, Yaffe MB, Clynes D, Moggs JG, Orphanides G, Thomson S, Edmunds JW, Clayton AL, Endicott JA, Mahadevan LC

Phosphorylation of histone H3 is implicated in transcriptional activation and chromosome condensation, but its immediate molecular function has remained obscure. By affinity chromatography of nuclear extracts against modified H3 tail peptides, we identified 14-3-3 isoforms as proteins that bind these tails in a strictly phosphorylation-dependent manner. Acetylation of lysines 9 and 14 does not impede 14-3-3 binding to serine 10-phosphorylated ... [more]

Mol. Cell Oct. 28, 2005; 20(2);199-211 [Pubmed: 16246723]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HIST1H3A YWHAG	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Klingberg R (2015)	Low	-	BioGRID	-
YWHAG HIST1H3A	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Macdonald N (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HIST1H3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

YWHAG