Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

RanBPM is expressed in synaptic layers of the mammalian retina and binds to metabotropic glutamate receptors.

Seebahn A, Rose M, Enz R

In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brain regions, including the retina. There, RanBPM is restricted to the inner plexiform layer where it co-localizes ... [more]

FEBS Lett. Jul. 09, 2008; 582(16);2453-7 [Pubmed: 18555800]

Throughput

  • High Throughput

Curated By

  • BioGRID