KCNIP3
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- calcium-dependent protein binding [ISO]
- ion channel binding [IPI]
- potassium channel regulator activity [IDA]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- calcium-dependent protein binding [ISO]
- ion channel binding [IPI]
- potassium channel regulator activity [IDA]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
SUMO1
Gene Ontology Biological Process
- PML body organization [ISO]
- negative regulation of DNA binding [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- palate development [ISO, ISS]
- positive regulation of calcium-transporting ATPase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein localization to nuclear pore [ISO]
- protein sumoylation [ISO, ISS]
- regulation of calcium ion transmembrane transport [ISO]
- regulation of cardiac muscle cell contraction [ISO]
- regulation of protein stability [ISO]
- regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Sumoylation regulates nuclear localization of repressor DREAM.
DREAM is a Ca(2+)-binding protein with specific functions in different cell compartments. In the nucleus, DREAM acts as a transcriptional repressor, although the mechanism that controls its nuclear localization is unknown. Yeast two-hybrid assay revealed the interaction between DREAM and the SUMO-conjugating enzyme Ubc9 and bioinformatic analysis identified four sumoylation-susceptible sites in the DREAM sequence. Single K-to-R mutations at positions ... [more]
Throughput
- Low Throughput
Additional Notes
- likely SUMO conjugate
Curated By
- BioGRID