MDM2
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- establishment of protein localization [ISO]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of protein processing [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- peptidyl-lysine modification [ISO]
- positive regulation of cell cycle [IGI]
- positive regulation of gene expression [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein export from nucleus [ISO]
- protein catabolic process [IDA]
- protein complex assembly [ISO]
- protein destabilization [ISO]
- protein localization to nucleus [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of protein catabolic process [ISO]
- traversing start control point of mitotic cell cycle [IDA]
Gene Ontology Molecular Function
USP7
Gene Ontology Biological Process
- histone deubiquitination [IBA]
- maintenance of DNA methylation [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of apoptotic process [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA, ISO]
- proteolysis [IMP]
- regulation of proteasomal protein catabolic process [IBA]
- regulation of protein stability [ISO]
- regulation of sequence-specific DNA binding transcription factor activity [ISO]
- regulation of transcription, DNA-templated [IBA]
- transcription-coupled nucleotide-excision repair [IBA, ISO]
Gene Ontology Molecular Function- cysteine-type endopeptidase activity [IMP, ISO]
- p53 binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin thiolesterase activity [IDA, ISO]
- ubiquitin-specific protease activity [IDA, ISO]
- cysteine-type endopeptidase activity [IMP, ISO]
- p53 binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin thiolesterase activity [IDA, ISO]
- ubiquitin-specific protease activity [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex.
The tumour suppressor p53, which accumulates in response to DNA damage and induces cell-cycle arrest and apoptosis, has a key function in the maintenance of genome integrity. Under normal conditions, the antiproliferative effects of p53 are inhibited by MDM2, a ubiquitin ligase that promotes p53 ubiquitination and degradation. MDM2 is also self-ubiquitinated and degraded. Here, we show that the tumour ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP7 MDM2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 590161 |
Curated By
- BioGRID