TRIM27
Gene Ontology Biological Process
- Arp2/3 complex-mediated actin nucleation [IDA]
- cell proliferation [TAS]
- innate immune response [IDA]
- interferon-gamma secretion [IMP]
- negative regulation of adaptive immune response [IMP]
- negative regulation of calcium ion import [IMP]
- negative regulation of gene expression, epigenetic [IDA]
- negative regulation of interleukin-2 secretion [IMP]
- negative regulation of protein kinase activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of tumor necrosis factor production [IMP]
- negative regulation of viral transcription [IDA]
- peptidyl-tyrosine phosphorylation [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- protein K63-linked ubiquitination [IMP]
- protein trimerization [IDA]
- retrograde transport, endosome to Golgi [IDA]
- spermatogenesis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE2I
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- protein ubiquitination [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SUMO E3 ligase activity of TRIM proteins.
SUMOylation governs numerous cellular processes and is essential to most eukaryotic life. Despite increasing recognition of the importance of this process, an extremely limited number of small ubiquitin-like modifier (SUMO) protein ligases (E3s) have been identified. Here we show that at least some members of the functionally diverse tripartite motif (TRIM) superfamily are SUMO E3s. These TRIM proteins bind both ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2I TRIM27 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | 4 | BioGRID | - |
Curated By
- BioGRID