Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Co-repressor activity of scaffold attachment factor B1 requires sumoylation.

Garee JP, Meyer R, Oesterreich S

Sumoylation is an emerging modification associated with a variety of cellular processes including the regulation of transcriptional activities of nuclear receptors and their coregulators. As SUMO modifications are often associated with transcriptional repression, we examined if sumoylation was involved in modulation of the transcriptional repressive activity of scaffold attachment factor B1. Here we show that SAFB1 is modified by both ... [more]

Biochem. Biophys. Res. Commun. May. 20, 2011; 408(4);516-22 [Pubmed: 21527249]

Throughput

  • Low Throughput

Additional Notes

  • source of PIAS1 not clear

Curated By

  • BioGRID