BAIT

BUD31

CWC14, YCR063W
Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
GO Process (3)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SNR7-S

U5S, SNR7
U5 spliceosomal RNA (U5 snRNA), short form; derived from SNR7-L by 3' end processing; associated with spliceosome
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

S.cerevisiae NineTeen complex (NTC) associated factor Bud31/Ycr063w assembles on pre-catalytic spliceosomes and improves first and second step pre-mRNA splicing efficiency.

Saha D, Khandelia P, O'Keefe RT, Vijayraghavan U

Pre-mRNA splicing occurs in spliceosomes whose assembly and activation is critical for splice site selection and catalysis. The highly conserved NTC protein complex stabilizes various snRNA and protein interactions early in the spliceosome assembly pathway. Among several NTC associated proteins is the non-essential protein Bud31/Ycr063w, which is also a component of the Cef1p sub-complex. A role for Bud31 in pre-mRNA ... [more]

Unknown Jan. 03, 2012; 0(0); [Pubmed: 22215661]

Throughput

  • Low Throughput

Curated By

  • BioGRID