BAIT
PCK1
PEPCK-C, PEPCK1, PEPCKC
phosphoenolpyruvate carboxykinase 1 (soluble)
GO Process (9)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- drug metabolic process [TAS]
- gluconeogenesis [ISS, TAS]
- glucose homeostasis [ISS]
- glucose metabolic process [IMP, TAS]
- glycerol biosynthetic process from pyruvate [ISS]
- internal protein amino acid acetylation [IDA]
- response to insulin [IDA]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
ATP2A1
ATP2A, SERCA1
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
GO Process (18)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- apoptotic mitochondrial changes [IMP]
- blood coagulation [TAS]
- calcium ion import [IMP]
- calcium ion transmembrane transport [IDA]
- calcium ion transport [IDA, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- ion transmembrane transport [TAS]
- maintenance of mitochondrion location [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IMP]
- negative regulation of striated muscle contraction [IMP]
- positive regulation of endoplasmic reticulum calcium ion concentration [IMP]
- positive regulation of fast-twitch skeletal muscle fiber contraction [IDA]
- positive regulation of mitochondrial calcium ion concentration [IMP]
- regulation of striated muscle contraction [IMP]
- relaxation of skeletal muscle [IDA]
- response to endoplasmic reticulum stress [IMP]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- H zone [IDA]
- I band [IDA]
- calcium channel complex [IC]
- endoplasmic reticulum membrane [IDA, TAS]
- endoplasmic reticulum-Golgi intermediate compartment [ISS]
- integral component of membrane [NAS]
- membrane [ISS]
- perinuclear region of cytoplasm [ISS]
- platelet dense tubular network membrane [TAS]
- sarcoplasmic reticulum [ISS, NAS]
- sarcoplasmic reticulum membrane [IC, TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Acetylation regulates gluconeogenesis by promoting PEPCK1 degradation via recruiting the UBR5 ubiquitin ligase.
Protein acetylation has emerged as a major mechanism in regulating cellular metabolism. Whereas most glycolytic steps are reversible, the reaction catalyzed by pyruvate kinase is irreversible, and the reverse reaction requires phosphoenolpyruvate carboxykinase (PEPCK1) to commit for gluconeogenesis. Here, we show that acetylation regulates the stability of the gluconeogenic rate-limiting enzyme PEPCK1, thereby modulating cellular response to glucose. High glucose ... [more]
Mol. Cell Jul. 08, 2011; 43(1);33-44 [Pubmed: 21726808]
Throughput
- High Throughput
Ontology Terms
- hek-293t cell (BTO:0002181)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID