STX1A
Gene Ontology Biological Process
- calcium ion-dependent exocytosis [ISO]
- intracellular protein transport [IBA]
- neurotransmitter transport [TAS]
- positive regulation of exocytosis [IDA]
- positive regulation of neurotransmitter secretion [IMP]
- regulation of exocytosis [IMP]
- response to gravity [IDA]
- secretion by cell [ISO]
- synaptic vesicle docking [IEP]
- synaptic vesicle fusion to presynaptic membrane [IBA]
Gene Ontology Molecular Function- ATP-dependent protein binding [IPI]
- SNAP receptor activity [IBA]
- SNARE binding [IDA, IPI, ISO]
- calcium channel inhibitor activity [ISO]
- calcium-dependent protein binding [IPI]
- chloride channel inhibitor activity [ISO]
- glycoprotein binding [IDA]
- kinase binding [ISO]
- myosin binding [IPI]
- myosin head/neck binding [IPI]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein binding, bridging [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- ATP-dependent protein binding [IPI]
- SNAP receptor activity [IBA]
- SNARE binding [IDA, IPI, ISO]
- calcium channel inhibitor activity [ISO]
- calcium-dependent protein binding [IPI]
- chloride channel inhibitor activity [ISO]
- glycoprotein binding [IDA]
- kinase binding [ISO]
- myosin binding [IPI]
- myosin head/neck binding [IPI]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein binding, bridging [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
Gene Ontology Cellular Component
- SNARE complex [ISO, ISS]
- actomyosin [IDA]
- cell [ISO]
- integral component of membrane [NAS]
- intracellular organelle [IDA]
- membrane [ISO]
- neuron projection [ISO]
- plasma membrane [IDA, ISO]
- protein complex [IDA]
- secretory granule [IDA]
- synaptic vesicle [IBA, ISO]
- synaptic vesicle membrane [ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [IDA]
VAMP2
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- calcium ion-dependent exocytosis [ISO]
- cellular response to insulin stimulus [ISO]
- eosinophil degranulation [ISO]
- long-term synaptic potentiation [ISO]
- membrane fusion [ISO]
- mucus secretion [ISO]
- natural killer cell degranulation [ISO]
- neurotransmitter secretion [IBA]
- neutrophil degranulation [ISO]
- positive regulation of intracellular protein transport [ISO]
- protein complex assembly [IDA]
- protein transport [IMP]
- regulation of exocytosis [IMP, ISO, TAS]
- regulation of histamine secretion by mast cell [ISO]
- regulation of vesicle-mediated transport [ISO]
- response to glucose [IDA]
- synaptic vesicle exocytosis [ISO]
- vesicle fusion [IBA]
- vesicle-mediated transport [IMP]
Gene Ontology Molecular Function- SNAP receptor activity [IBA]
- SNARE binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- identical protein binding [IPI]
- myosin binding [IPI]
- phospholipid binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [IDA, IPI]
- SNAP receptor activity [IBA]
- SNARE binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- identical protein binding [IPI]
- myosin binding [IPI]
- phospholipid binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [IDA, IPI]
Gene Ontology Cellular Component
- SNARE complex [IDA, ISO]
- cell [ISO]
- clathrin-coated vesicle [ISO, ISS]
- cytoplasmic vesicle [ISO]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA]
- intracellular organelle [IDA]
- membrane [ISO, ISS]
- neuron projection [IDA]
- neuron projection terminus [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- secretory granule [IDA, ISO]
- secretory granule membrane [ISO]
- synapse [ISO]
- synaptic vesicle [IBA, IDA, ISO]
- synaptic vesicle membrane [ISO, NAS]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [IDA]
- terminal bouton [IDA]
- trans-Golgi network [ISO]
- zymogen granule membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SUMOylation regulates insulin exocytosis downstream of secretory granule docking in rodents and humans.
The reversible attachment of small ubiquitin-like modifier (SUMO) proteins controls target localization and function. We examined an acute role for the SUMOylation pathway in downstream events mediating insulin secretion.We studied islets and β-cells from mice and human donors, as well as INS-1 832/13 cells. Insulin secretion, intracellular Ca(2+), and β-cell exocytosis were monitored after manipulation of the SUMOylation machinery. Granule ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX1A VAMP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STX1A VAMP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID