BAIT
SUMO3
small ubiquitin-like modifier 3
GO Process (3)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
RAP1A
RAP-1A, rap 1A
RAP1A, member of RAS oncogene family
GO Process (15)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- GTP catabolic process [ISO]
- Rap protein signal transduction [ISO, ISS]
- cellular response to cAMP [ISO, ISS]
- cellular response to nerve growth factor stimulus [IDA]
- establishment of endothelial barrier [ISO, ISS]
- negative regulation of synaptic vesicle exocytosis [ISO]
- nerve growth factor signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISO]
- positive regulation of Rap GTPase activity [IMP]
- positive regulation of neuron projection development [IMP, ISO]
- positive regulation of protein kinase activity [IMP]
- positive regulation of vasculogenesis [ISO, ISS]
- protein transport [ISO, ISS]
- regulation of cell junction assembly [ISO, ISS]
- small GTPase mediated signal transduction [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Analysis of Oxygen/Glucose-Deprivation-Induced Changes in SUMO3 Conjugation Using SILAC-Based Quantitative Proteomics.
Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on SUMO conjugation of target proteins, we exposed neuroblastoma B35 cells expressing HA-SUMO3 to transient OGD and used ... [more]
J. Proteome Res. Dec. 01, 2011; 0(0); [Pubmed: 22082260]
Throughput
- High Throughput
Curated By
- BioGRID