An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Analysis of Oxygen/Glucose-Deprivation-Induced Changes in SUMO3 Conjugation Using SILAC-Based Quantitative Proteomics.

Yang W, Thompson JW, Wang Z, Wang L, Sheng H, Foster MW, Moseley MA, Paschen W

Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on SUMO conjugation of target proteins, we exposed neuroblastoma B35 cells expressing HA-SUMO3 to transient OGD and used ... [more]

J. Proteome Res. Dec. 01, 2011; 0(0); [Pubmed: 22082260]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

SUMO3

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO transferase activity [ISO]

Gene Ontology Cellular Component

COX2