An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic Dissection of the von Hippel-Lindau (VHL) Interactome.

Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y

The von Hippel-Lindau (VHL) tumor suppressor gene encodes a component of a ubiquitin ligase complex containing elongin B, elongin C, cullin 2, and Rbx1, which acts as a negative regulator of hypoxia inducible factor (HIF). VHL ubiquitinates and degrades the alpha subunits of HIF, and this is proposed to suppress tumorigenesis and tumor angiogenesis. Several lines of evidence also suggest ... [more]

J. Proteome Res. Nov. 04, 2011; 10(11);5175-82 [Pubmed: 21942715]

Throughput

High Throughput

Additional Notes

FLAG-VHL19 interacting proteins
FLAG-VHL30 interacting proteins

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VHL CCT6A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Chesnel F (2020)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

VHL

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]protein binding [IPI]transcription factor binding [IPI]ubiquitin protein ligase activity [IDA]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

CCT6A

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]