YWHAQ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EXO1
Gene Ontology Biological Process
Gene Ontology Molecular Function- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
14-3-3 checkpoint regulatory proteins interact specifically with DNA repair protein human exonuclease 1 (hEXO1) via a semi-conserved motif.
Human exonuclease 1 (hEXO1) acts directly in diverse DNA processing events, including replication, mismatch repair (MMR), and double strand break repair (DSBR), and it was also recently described to function as damage sensor and apoptosis inducer following DNA damage. In contrast, 14-3-3 proteins are regulatory phosphorserine/threonine binding proteins involved in the control of diverse cellular events, including cell cycle checkpoint ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
YWHAQ EXO1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3376296 | |
EXO1 YWHAQ | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
YWHAQ EXO1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3536314 | |
YWHAQ EXO1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID