An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.

Ha K, Fiskus W, Rao R, Balusu R, Venkannagari S, Nalabothula NR, Bhalla KN

Following DNA damage that results in stalled replication fork, activation of ATR-CHK1 signaling induces the DNA damage response (DDR) in transformed cells. In the present studies on human cervical and breast cancer cells, we determined the effects of hsp90 inhibition on the levels and accumulation of DNA damage/repair-associated proteins following exposure to Î³-ionizing radiation (IR; 4 Gy). We show that ... [more]

Mol. Cancer Ther. Jul. 01, 2011; 10(7);1194-206 [Pubmed: 21566061]

Throughput

Low Throughput

Ontology Terms

hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Curated By

BioGRID

Interaction Summary

ATR

Gene Ontology Biological Process

Gene Ontology Molecular Function

MutLalpha complex binding [IDA]MutSalpha complex binding [IDA]protein binding [IPI]protein dimerization activity [IBA]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

HSPA4