BAIT

ELP4

HAP1, KTI9, TOT7, Elongator subunit ELP4, YPL101W
Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

TK(UUU)K

L000003638
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Elongator complex influences telomeric gene silencing and DNA damage response by its role in wobble uridine tRNA modification.

Chen C, Huang B, Eliasson M, Ryden P, Bystroem AS

Elongator complex is required for formation of the side chains at position 5 of modified nucleosides 5-carbamoylmethyluridine (ncm⁵U₃₄), 5-methoxycarbonylmethyluridine (mcm⁵U₃₄), and 5-methoxycarbonylmethyl-2-thiouridine (mcm⁵s²U₃₄) at wobble position in tRNA. These modified nucleosides are important for efficient decoding during translation. In a recent publication, Elongator complex was implicated to participate in telomeric gene silencing and DNA damage response by interacting with proliferating ... [more]

PLoS Genet. Sep. 01, 2011; 7(9);e1002258 [Pubmed: 21912530]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: vegetative growth (APO:0000106)
  • phenotype: silencing (APO:0000046)
  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • Elevated levels of some modified tRNAs suppress the defects in telomeric silencing and DNA damage response seen in Elongator complex mutants

Curated By

  • BioGRID