BAIT

SLC14A2

Slc14a2T, Slc14a2_v4, UrT1-C, UrT1-D, testSymbol
solute carrier family 14 (urea transporter), member 2
GO Process (3)
GO Function (3)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rattus norvegicus
PREY

UBC

ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MDM2 E3 ubiquitin ligase mediates UT-A1 urea transporter ubiquitination and degradation.

Chen G, Huang H, Froehlich O, Yang Y, Klein JD, Price SR, Sands JM

UT-A1 is the primary urea transporter in the apical plasma membrane responsible for urea reabsorption in the inner medullary collecting duct. Although the physiological function of UT-A1 has been well established, the molecular mechanisms that regulate its activity are less well understood. Analysis of the UT-A1 amino acid sequence revealed a potential MDM2 E3 ubiquitin ligase-binding motif in the large ... [more]

Am. J. Physiol. Renal Physiol. Nov. 01, 2008; 295(5);F1528-34 [Pubmed: 18784257]

Throughput

  • Low Throughput

Curated By

  • BioGRID