BAIT

MED8

L000003917, YBR193C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Saccharomyces cerevisiae (S288c)
PREY

RCO1

YMR075W
Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Histone modifications influence mediator interactions with chromatin.

Zhu X, Zhang Y, Bjornsdottir G, Liu Z, Quan A, Costanzo M, Davila Lopez M, Westholm JO, Ronne H, Boone C, Gustafsson CM, Myers LC

The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silenced locations. We provide evidence that Mediator localization is guided by an interaction with histone tails, and that ... [more]

Nucleic Acids Res. Oct. 01, 2011; 39(19);8342-54 [Pubmed: 21742760]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • interaction determined by Random Spore Analysis (RSA)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RCO1 MED8
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.4273BioGRID
221002

Curated By

  • BioGRID