BAIT

MED8

L000003917, YBR193C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

GO Process (2)

GO Function (4)

GO Component (1)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IDA]

positive regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II transcription corepressor activity [IPI]
TBP-class protein binding [IDA]
structural molecule activity [IMP]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

MAC1

CUA1, L000000973, L000001035, YMR021C

Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS

GO Process (3)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

cellular copper ion homeostasis [IMP]

positive regulation of protein catabolic process [IMP]

positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
core promoter proximal region DNA binding [IDA]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Histone modifications influence mediator interactions with chromatin.

Zhu X, Zhang Y, Bjornsdottir G, Liu Z, Quan A, Costanzo M, Davila Lopez M, Westholm JO, Ronne H, Boone C, Gustafsson CM, Myers LC

The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silenced locations. We provide evidence that Mediator localization is guided by an interaction with histone tails, and that ... [more]

Nucleic Acids Res. Oct. 01, 2011; 39(19);8342-54 [Pubmed: 21742760]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

interaction determined by Random Spore Analysis (RSA)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MED8 MAC1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1652	BioGRID	1962106

Curated By

BioGRID

Interaction Summary

MED8

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II transcription corepressor activity [IPI]TBP-class protein binding [IDA]structural molecule activity [IMP]

Gene Ontology Cellular Component

MAC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]core promoter proximal region DNA binding [IDA]

Gene Ontology Cellular Component

Synthetic Growth Defect

Publication

Histone modifications influence mediator interactions with chromatin.

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II transcription corepressor activity [IPI]
TBP-class protein binding [IDA]
structural molecule activity [IMP]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
core promoter proximal region DNA binding [IDA]